The mechanism behind oil synthesis within microalgae cells has been revealed by a Japanese research team. This discovery could contribute to the development of biofuels. The findings were published on April 4 in Scientific Reports.

The research was carried out by a group led by Professor HASUNUMA Tomohisa and Academic Researcher KATO Yuichi, both from the Kobe University Graduate School of Science, Technology and Innovation.

During the 20th century the petrochemical industry developed rapidly, leading to depletion of fossil resources and climate change on a global scale. In order to solve these issues and realize a sustainable and environmentally-conscious society, we must make use of renewable biomass such as plants and algae.

The amount of biomass on Earth is approximately 10 times the amount of energy we currently consume. Roughly half of this biomass grows in aquatic environments, and ocean-based biomass such as microalgae can produce oil without using up arable land and drinking water.

Microalgae can grow with light, water, carbon dioxide and a small amount of minerals, and their cells divide quickly, meaning that they can be harvested faster than land-based biomasses. Algae can also be harvested all year round, potentially offering a more stable energy supply.

Many species of algae are capable of producing large amounts of oil (lipids), but this is the first time that researchers have captured the metabolic changes occurring on a molecular level when lipids are produced in algae cells.

Focusing on marine microalgae, Professor Hasunuma's group found that Chlamydomonas sp. JSC4, a new species of green alga harvested from brackish water, combines a high growth rate with high levels of lipids. The research team developed an analysis method called "dynamic metabolic profiling" and used this to analyze JSC4 and discover how this species produces oil within its cells.

Professor Hasunuma's team incubated JSC4 with carbon dioxide as the sole carbon source. 4 days after the start of incubation, over 55% of cell weight consisted of carbohydrates (mainly starch). When saltwater comprised 1-2% of the incubation liquid, the team saw a decrease in carbohydrates and increase in oil, and 7 days after the start of incubation over 45% of cell weight had become oil.

JSC4 has a high cell growth rate, and the lipid production rate in the culture solution achieved a speed that greatly surpassed previous experiments. At the start of the cultivation period starch particles were observed in the cells, but in saltwater these particles vanish and numerous oil droplets are seen (figure 1).

Using dynamic metabolic profiling, the group found that the sugar biosynthesis pathway (activated when starch is produced) slows down, and the pathway is activated for synthesizing triacylglycerol, a constituent element of oil. In other words, the addition of seawater switched the pathway from starch to oil production. They also clarified that the activation of an enzyme that breaks down starch is increased in saltwater solution.

The discovery of this metabolic mechanism is not only an important biological finding, it could also be used to increase the production of biofuel by improving methods of algae cultivation. Based on these findings, the team will continue looking for ways to increase sustainable oil production by developing more efficient cultivation methods and through genetic engineering.

Research paper

Tweet

Algal residue - an alternative carbon resource for pharmaceuticals and polyesters
Tokyo, Japan (SPX) Apr 13, 2017
Microalgae have received much attention in biomass production due to many strains having a high biomass productivity per unit time and per unit area. Algae produce high levels of oil as well as carbohydrates, occurring mainly in the form of starch. They can survive in unfavorable, nutrient deficient conditions, and can be propagated industrially without using farmland. Oil derived from alg ... read more

Related Links
Kobe University
Bio Fuel Technology and Application News

Thanks for being here; We need your help. The SpaceDaily news network continues to grow but revenues have never been harder to maintain. With the rise of Ad Blockers, and Facebook - our traditional revenue sources via quality network advertising continues to decline. And unlike so many other news sites, we don't have a paywall - with those annoying usernames and passwords. Our news coverage takes time and effort to publish 365 days a year. If you find our news sites informative and useful then please consider becoming a regular supporter or for now make a one off contribution.
SpaceDaily Contributor $5 Billed Once credit card or paypal		SpaceDaily Monthly Supporter $5 Billed Monthly paypal only